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<h1>siRNA干擾檢測服務(wù)</h1> <div id="im8tiuhso" class="jyms"><p><span>簡(jiǎn)要描述：</span>RNA干擾 (RNA interference, RNAi)技術(shù)，是將與內源mRNA 編碼區同源的小片段(19 -23bp)外源雙鏈 RNA(siRNA)導入細胞中，引發(fā)細胞中相應mRNA 高效特異性降解，從而導致特定基因表達沉默(knock down)的一種實(shí)驗技術(shù)。</p></div> <ul> <li><img src="/Skins/270236/images/dot.png" /><span>產(chǎn)品型號：</span></li> <li><img src="/Skins/270236/images/dot.png" /><span>廠(chǎng)商性質(zhì)：</span>生產(chǎn)廠(chǎng)家</li> <li><img src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪(fǎng) 問(wèn) 量：</span>3174</li> </ul> <div id="im8tiuhso" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="im8tiuhso" class="clear"></div> </div>  <div class="im8tiuhso" id="ny_pro_box02"> <div class="im8tiuhso" id="con"> <ul id="tags"> <li id="im8tiuhso" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="im8tiuhso" id=tagContent> <div id="im8tiuhso" class="tagContent selectTag" id=tagContent0><p>siRNA干擾檢測服務(wù)</p><p>原理：RNA干擾 (RNA interference, RNAi)技術(shù)，是將與內源mRNA 編碼區同源的小片段(19 -23bp)外源雙鏈 RNA(siRNA)導入細胞中，引發(fā)細胞中相應mRNA 高效特異性降解，從而導致特定基因表達沉默(knock down)的一種實(shí)驗技術(shù)，其中通過(guò)構建表達載體表達siRNA是多種siRNA制備方法中*有可能進(jìn)行長(cháng)期基因沉默研究的方法，siRNA 載體可以在細胞內直接轉錄出shrna</a> ，其干擾效果等同于siRNA ，而且能夠解決 siRNA干擾時(shí)間短的缺點(diǎn)。您只需提供需干擾基因的詳細信息，包括基因的 mRNA 序列，Genbank Accession No. 和所屬物種，本公司負責設計3條針對目的mRNA的siRNA 靶序列，在短時(shí)間內構建三個(gè)可用于目的mRNA干擾實(shí)驗的siRNA 載體并為您進(jìn)行后續的干擾實(shí)驗研究。</p><p>實(shí)驗流程</p><p>◇ 選擇siRNA表達載體通過(guò)人工合成基因的方法將該序列克隆至Clontech公司的RNAi-Ready pSIREN系列表達載體中，該系列表達載體帶有Pol III(U6)啟動(dòng)子，具有轉染效率高、操作簡(jiǎn)單、rna沉默</a>效果易于檢測等特點(diǎn)，客戶(hù)可根據需要進(jìn)行選擇。</p><p>◇ 設計siRNA</a>靶序列</p><p>◇ 根據客戶(hù)提交的目的mRNA序列，設計3條RNA干擾</a>靶序列，靶序列一般為19-21nt 長(cháng)。</p><p>◇ 對于每條選定的siRNA 靶序列，設計 siRNA 正義鏈和反義鏈，以 loop(9nt or 10nt) 相連，形成穩定的發(fā)卡RNA，稱(chēng)為 shRNA(short hairpin RNA)。</p><p>◇ 合成模板合成每條編碼 shRNA的DNA 模板，并連接到 siRNA 載體相應的多克隆位點(diǎn)之間。連接產(chǎn)物轉化DH5α大腸桿菌</a>。</p><p>◇ 抽提陽(yáng)性克隆質(zhì)粒公司試劑盒</a>抽提陽(yáng)性克隆載體并定量。</p><p>◇ 轉染細胞質(zhì)粒直接轉染或通過(guò)病毒包裝后轉染細胞，并對轉染細胞進(jìn)行陽(yáng)性鑒定和轉代細胞株的培養。</p><p>◇ 干擾效果檢測</p><p>◇ 基因水平上檢測提取RNA、反轉錄、Realtime PCR檢測干擾效果(客戶(hù)自備細胞系)。</p><p>◇ 蛋白水平上檢測 Western Blot檢測蛋白水平干擾效果(客戶(hù)自備一抗)。</p><p>服務(wù)周期及價(jià)格</p><table border="1" cellpadding="0" cellspacing="0"><tbody><tr><td><p>服務(wù)內容</p></td><td><p>價(jià)格(￥)</p></td><td><p>周期</p></td></tr><tr><td><p>siRNA 載體構建1～3個(gè)</p></td><td><p>1800元/個(gè)</p></td><td><p>14個(gè)工作日</p></td></tr><tr><td><p>siRNA 載體構建3～10個(gè)</p></td><td><p>1500元/個(gè)</p></td><td><p>20個(gè)工作日</p></td></tr><tr><td><p>siRNA 載體構建10個(gè)以上</p></td><td><p>詢(xún)價(jià)</p></td><td><p>協(xié)商</p></td></tr><tr><td><p>細胞培養、轉染</p></td><td><p>5000元/基因</p></td><td><p>1～2周</p></td></tr><tr><td><p>基因水平上檢測干擾效果</p></td><td><p>詢(xún)價(jià)（視檢測數量商定）</p></td><td><p>5～6周</p></td></tr><tr><td><p>蛋白水平上檢測干擾效果</p></td><td><p>詢(xún)價(jià)（視檢測數量商定）</p></td><td><p>5～6周</p></td></tr></tbody></table><p>服務(wù)說(shuō)明</p><p>◇ siRNA服務(wù)需客戶(hù)與我們共同討論實(shí)驗方案，并提供靶基因名稱(chēng)、序列或GeneBank ID和希望插入載體的名稱(chēng)等，免費為您設計siRNA序列。</p><p>◇ 由于構建的siRNA表達載體中插入的片段可以形成hairpin結構，因此可能會(huì )影響測序。如果我們對質(zhì)粒測序結果不理想時(shí)，將對由質(zhì)粒擴增得到的PCR產(chǎn)物進(jìn)行測序。</p><p>◇ 開(kāi)始實(shí)驗時(shí)公司和客戶(hù)簽訂詳細的實(shí)驗技術(shù)服務(wù)合同、保密合同，客戶(hù)需預付合同金額的50%作為預付款。</p><p>提供結果</p><p>實(shí)驗報告包括：siRNA序列、構建載體的測序報告、熒光定量PCR檢測報告、Western Blot圖片及全部實(shí)驗數據和詳細的實(shí)驗步驟。</p><p> </p></div> <div class="im8tiuhso" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <a name="order" id="order"></a> <div id="im8tiuhso" class="ly_msg" 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