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id="im8tiuhso" class="about"> <div id="im8tiuhso" class="PageView"> <div class="im8tiuhso" id="nr_main_box">  <div id="im8tiuhso" class="pro_detail_main"> <div id="im8tiuhso" class="prodetail_img"> <div class="im8tiuhso" id="preview"> <div id="im8tiuhso" class="jqzoom" id="spec-n1"> <div class="im8tiuhso" id="ceshi" style=""> <img src="http://img75.chem17.com/gxhpic_33572a5ad1/8975028d8e14767f18d78b6f70be0028ea71f0fbbb8c674025c7db15d44444fc2ebb78827fdc69db.png" jqimg="http://img75.chem17.com/gxhpic_33572a5ad1/8975028d8e14767f18d78b6f70be0028ea71f0fbbb8c674025c7db15d44444fc2ebb78827fdc69db.png" /> </div> </div> <div class="im8tiuhso" id="spec-n5">  <div class="im8tiuhso" id="spec-list"> <ul class="list-h" > <li><p><img onclick="jQuery('.videoItem').hide();mybaidu.window.SetPause();" src="http://img75.chem17.com/gxhpic_33572a5ad1/8975028d8e14767f18d78b6f70be0028ea71f0fbbb8c674025c7db15d44444fc2ebb78827fdc69db_800_800_5.png" alt="" data-zoom-image="http://img75.chem17.com/gxhpic_33572a5ad1/8975028d8e14767f18d78b6f70be0028ea71f0fbbb8c674025c7db15d44444fc2ebb78827fdc69db_800_800_5.png" onload="DrawImage(this,460,300)"></p></li> </ul> </div>  </div> </div> </div> <div id="im8tiuhso" class="pro_detail_text"> <h1>質(zhì)粒DNA抽提</h1> <div id="im8tiuhso" class="jyms"><p><span>簡(jiǎn)要描述：</span>質(zhì)粒DNA抽提通常是指從大腸桿菌中進(jìn)行小量質(zhì)粒的快速抽提的方法，通過(guò)試劑盒采用新型的離子交換柱，在特定條件下，使質(zhì)粒能在離心過(guò)柱的瞬間，結合到質(zhì)粒純化柱上，在一定條件下又能將質(zhì)粒充分洗脫，從而實(shí)現質(zhì)粒的快速純化。無(wú)需酚氯仿抽提，無(wú)需酒精沉淀，12個(gè)樣品只需不足30分鐘即可完成。</p></div> <ul> <li><img src="/Skins/270236/images/dot.png" /><span>產(chǎn)品型號：</span></li> <li><img src="/Skins/270236/images/dot.png" /><span>廠(chǎng)商性質(zhì)：</span>生產(chǎn)廠(chǎng)家</li> <li><img src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪(fǎng) 問(wèn) 量：</span>2603</li> </ul> <div id="im8tiuhso" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="im8tiuhso" class="clear"></div> </div>  <div class="im8tiuhso" id="ny_pro_box02"> <div class="im8tiuhso" id="con"> <ul id="tags"> <li id="im8tiuhso" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="im8tiuhso" id=tagContent> <div id="im8tiuhso" class="tagContent selectTag" id=tagContent0><p>質(zhì)粒DNA抽提:</p><p> 通常是指從大腸桿菌中進(jìn)行小量質(zhì)粒的快速抽提的方法，通過(guò)試劑盒采用新型的離子交換柱，在特定條件下，使質(zhì)粒能在離心過(guò)柱的瞬間，結合到質(zhì)粒純化柱上，在一定條件下又能將質(zhì)粒充分洗脫，從而實(shí)現質(zhì)粒的快速純化。無(wú)需酚氯仿抽提，無(wú)需酒精沉淀，12個(gè)樣品只需不足30分鐘即可完成。</p><p>質(zhì)粒DNA抽提效率:<br /> 1.每個(gè)質(zhì)粒純化柱可以結合的質(zhì)粒量的上限約為20微克。</p><p> 2.每個(gè)純化柱可用于抽提1-5毫升用LB培養的大腸桿菌。</p><p> 3.抽提所得質(zhì)粒的OD值一般在1.80左右。</p><p> 4.抽提獲得的質(zhì)粒量會(huì )受質(zhì)?？截悢档纫蛩赜绊?。</p><p> 5.抽提獲得的質(zhì)粒DNA的OD值也會(huì )因菌種不同等原因而略有波動(dòng)。</p><p>用途：<br /> 質(zhì)?？芍苯佑糜谵D染細胞，DNA測序，PCR，基于PCR的突變，體外轉錄，轉化細菌，內切酶消化等。</p><p><br />所需試劑：溶液I（懸浮液），溶液II（裂解液），溶液III（結合液），溶液IV（洗滌液），溶液V（洗脫液），RNase A（100mg/ml），質(zhì)粒純化柱，廢液收集管等</p><p>保存條件：<br /> 室溫保存，一年有效。</p><p><br />注意事項： <br /> 1.*次使用前把試劑盒提供的RNase A全部加到溶液I（懸浮液）中，混勻，并在瓶上做好標記。加入RNase A后4℃存放。<br /> 2.*次使用前在每瓶溶液IV（洗滌液）中加入54ml無(wú)水乙醇，混勻，并在瓶上做好標記。<br /> 3.溫度較低時(shí)，溶液II和溶液III可能會(huì )有沉淀產(chǎn)生。使用前必須檢查一遍。如有沉淀，37℃水浴加熱溶解，混勻后使用。溶液II請勿過(guò)分劇烈混勻，否則會(huì )產(chǎn)生大量氣泡。<br /> 4.溶液II使用完后，一定要蓋緊瓶蓋，防止被空氣中二氧化碳酸化。<br /> 5.溶液II有強堿性，溶液II和溶液III對人體都有刺激性，操作時(shí)請小心，并注意適當防護。<br /> 6.所有操作均在室溫進(jìn)行，操作時(shí)無(wú)需冰浴。所有離心也均在室溫進(jìn)行。<br /> 7.廢液收集管在一次抽提中需多次使用，切勿中途丟棄。<br /> 8.為了安全和健康，請穿實(shí)驗服并戴一次性手套操作。</p></div> <div class="im8tiuhso" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <a name="order" id="order"></a> <div id="im8tiuhso" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 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