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jqimg="http://img55.chem17.com/gxhpic_33572a5ad1/6fd65d789e787a6b24380909b8d2cf971d976dfdd26c503305a7578aecbf65403c84e849be2c3603.jpg" /> </div> </div> <div class="im8tiuhso" id="spec-n5">  <div class="im8tiuhso" id="spec-list"> <ul class="list-h" > <li><p><img onclick="jQuery('.videoItem').hide();mybaidu.window.SetPause();" src="http://img55.chem17.com/gxhpic_33572a5ad1/6fd65d789e787a6b24380909b8d2cf971d976dfdd26c503305a7578aecbf65403c84e849be2c3603_800_800_5.jpg" alt="" data-zoom-image="http://img55.chem17.com/gxhpic_33572a5ad1/6fd65d789e787a6b24380909b8d2cf971d976dfdd26c503305a7578aecbf65403c84e849be2c3603_800_800_5.jpg" onload="DrawImage(this,460,300)"></p></li> </ul> </div>  </div> </div> </div> <div id="im8tiuhso" class="pro_detail_text"> <h1>Multiplex SNaPshot SNP分型法</h1> <div id="im8tiuhso" class="jyms"><p><span>簡(jiǎn)要描述：</span>SNP（single nucleotide polymorphism），即單核苷酸多態(tài)，是由于單個(gè)核苷酸改變而導致的核酸序列多態(tài)。一般來(lái)說(shuō)，SNP在人基因組中的發(fā)生頻率比較高，大約平均每1000個(gè)堿基中就有一個(gè)多態(tài)位點(diǎn)，是繼微衛星之后的第三代遺傳標記。SNP廣泛用于群體遺傳學(xué)研究（如生物的起源、進(jìn)化及遷移等方面）和疾病相關(guān)基因的研究，在藥物基因學(xué)、診斷學(xué)和生物醫學(xué)研究中起重要作用。</p></div> <ul> <li><img src="/Skins/270236/images/dot.png" /><span>產(chǎn)品型號：</span></li> <li><img src="/Skins/270236/images/dot.png" /><span>廠(chǎng)商性質(zhì)：</span>生產(chǎn)廠(chǎng)家</li> <li><img src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪(fǎng) 問(wèn) 量：</span>3781</li> </ul> <div id="im8tiuhso" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="im8tiuhso" class="clear"></div> </div>  <div class="im8tiuhso" id="ny_pro_box02"> <div class="im8tiuhso" id="con"> <ul id="tags"> <li id="im8tiuhso" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="im8tiuhso" id=tagContent> <div id="im8tiuhso" class="tagContent selectTag" id=tagContent0><p>SNP（single nucleotide polymorphism）原理</p><p> SNP（single nucleotide polymorphism），即單核苷酸多態(tài)，是由于單個(gè)核苷酸改變而導致的核酸序列多態(tài)。一般來(lái)說(shuō)，一個(gè)SNP位點(diǎn)只有兩種等位基因，因此又叫雙等位基因。SNP在人基因組中的發(fā)生頻率比較高，大約平均每1000個(gè)堿基中就有一個(gè)多態(tài)位點(diǎn)，是繼微衛星之后的第三代遺傳標記。有些SNP位點(diǎn)還會(huì )影響基因的功能，從而導致生物性狀改變甚至致病。SNP廣泛用于群體遺傳學(xué)研究（如生物的起源、進(jìn)化及遷移等方面，將逐漸取代微衛星而成為新一代的分子生物學(xué)標記）和疾病相關(guān)基因的研究，在藥物基因組學(xué)、診斷學(xué)和生物醫學(xué)研究中起重要作用。</p><p>Multiplex SNaPshot SNP分型法</p><p>SNaPshot SNP分型法</p><p> 是基于熒光標記單堿基延伸原理的分型技術(shù)，也稱(chēng)小測序，主要針對中等通量的SNP分型項目。在一個(gè)含有測序酶、四種熒光標記ddNTP、緊臨多態(tài)位點(diǎn)5’-端的不同長(cháng)度延伸引物和PCR產(chǎn)物模板的反應體系中，引物延伸一個(gè)堿基即終止，經(jīng)ABI測序儀檢測后，根據峰的移動(dòng)位置確定該延伸產(chǎn)物對應的SNP位點(diǎn)，根據峰的顏色可得知摻入的堿基種類(lèi)，從而確定該樣本的基因型。對于PCR產(chǎn)物模板可通過(guò)多重PCR反應體系來(lái)獲得。通常用于10-30個(gè)SNP位點(diǎn)分析。</p><p>SNaPshot SNP分型法服務(wù)流程</p><p> 1. 客戶(hù)提供：</p><p> 1）.已純化基因組DNA，并填寫(xiě)詳細基因組DNA量，濃度及純度</p><p> 2）.或提供新鮮的組織，血液，細胞樣本；且盡量多的材料</p><p> 3）.提供已知的基因序列或物種基因序列號；盡可能詳細的背景資料。</p><p>2.設計PCR 擴增含SNPs 位點(diǎn)的一段DNA序列。</p><p>3.對PCR 產(chǎn)物進(jìn)行純化（去除引物和dNTP）。</p><p>4.引物延伸。</p><p>5.延伸產(chǎn)物測序。</p><p>6.提供：峰圖文件（abl.格式），序列文件（seq.格式）；正式的數據分析報告。</p><p><img alt="" src="https://img51.chem17.com/gxhpic_33572a5ad1/34139391ea0c7f8fbbc9db1a4d4515276c0782b4f9715c23690987bf7c9b219a052bc1737dce533e.jpg" /></p><p>樣本運輸：</p><p> 客戶(hù)根據溫度，所在區域及樣本類(lèi)型的差異，以加冰袋或干冰運輸，并保證樣本的密封性和完整性，以免造成不必要的污染和損耗。</p></div> <div class="im8tiuhso" id="nr_textbox"><link rel="stylesheet" type="text/css" 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