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/><span>更新時(shí)間：</span>2024-05-15</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪(fǎng) 問(wèn) 量：</span>9109</li> </ul> <div id="im8tiuhso" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="im8tiuhso" class="clear"></div> </div>  <div class="im8tiuhso" id="ny_pro_box02"> <div class="im8tiuhso" id="con"> <ul id="tags"> <li id="im8tiuhso" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="im8tiuhso" id=tagContent> <div id="im8tiuhso" class="tagContent selectTag" id=tagContent0><p>微衛星（STR）檢測</p><p><strong>STR檢測服務(wù)</strong>STR原理：</p><p>短串聯(lián)重復序列（short tandem repeat, STR），也稱(chēng)微衛星 DNA（microsalite DNA），是由2 - 6個(gè)堿基對作為核心單位，串聯(lián)重復形成的一類(lèi)DNA序列，由于核心單位重復數目在個(gè)體間呈高度變異性并且數量豐富，構成了STR基因座的遺傳多態(tài)性。它分布于人類(lèi)整個(gè)基因組，平均每15 kb就存在一個(gè)STR基因座。人類(lèi)基因組內已發(fā)現了七千個(gè)以上的STR位點(diǎn)。它具有分布廣泛，易于檢測，信息量大，有高度多態(tài)性并遵循孟德?tīng)柟诧@性遺傳等優(yōu)點(diǎn)。是繼限制性片段長(cháng)度多態(tài)性（RFLP）之后的第二代遺傳記，目前STR分析已廣泛應用于遺傳制圖、連鎖性分析、法醫學(xué)鑒定、疾病基因定位、物種多態(tài)性和遺傳病的診斷等諸多領(lǐng)域。</p><p><strong style="text-wrap: wrap;">STR檢測服務(wù)</strong>流程：</p><p>1. 客戶(hù)提供</p><p>A.如果提供基因組DNA：至少提供100 ng DNA樣品（針對每個(gè)多態(tài)性位點(diǎn)，需樣品3-5 ul，濃度為30 - 50 ng/ul）。</p><p>B.如果提供PCR產(chǎn)物：產(chǎn)物要通過(guò)預實(shí)驗的電泳檢測，特異性要好。</p><p>C.如果需要設計引物，請提供目的片段序列或者微衛星檢測位點(diǎn)，引物設計好后需要客戶(hù)確認。</p><p>D.除提供樣品類(lèi)型，DNA濃度和純度值外，還需要注明可能殘留的抑制劑成分。樣品溶解在ddH2O中，如果不是，請注明溶解液成分。</p><p>2. 確定STR位點(diǎn)，設計實(shí)驗方案，包括熒光標記的選擇，引物的設計、合成和標記，熒光分子量?jì)葮说倪x用等。</p><p>3. 按照所設計的實(shí)驗方案完成熒光PCR，利用瓊脂糖電泳檢測結果；若PCR產(chǎn)物特異性不夠，將進(jìn)行優(yōu)化。</p><p>4. PCR產(chǎn)物通過(guò)ABI 3730XL 測序儀器進(jìn)行毛細管電泳檢測，獲得擴增片段大小的數據。</p><p>5. 用GeneScan 3.0/GenoTyper 2.0或GeneMapper 3.2等軟件對收集到的數據進(jìn)行處理分析，將產(chǎn)物片段大小、數量多少的信息轉化成直觀(guān)準確的波形圖譜，為下一步進(jìn)行關(guān)聯(lián)分析或連鎖分析等遺傳分析提供可靠的數據。</p><p>6.發(fā)送準確的微衛星（STR）分型結果報告及PCR產(chǎn)物。<img alt="STR檢測服務(wù)"src="https://img55.chem17.com/34139391ea0c7f8fbbc9db1a4d451527bcdc65a69b7bfe9648e9e3fe5f457219c2ed932f35179afb.jpg"/></p><p><br/></p></div> <div class="im8tiuhso" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <a name="order" id="order"></a> <div id="im8tiuhso" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="im8tiuhso" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="STR檢測服務(wù)" placeholder="請輸入產(chǎn)品名稱(chēng)" /></div> </li> <li> <h4>您的單位：</h4> <div id="im8tiuhso" class="msg_ipt12"><input class="textborder" size="42" 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id="bvvvf"></strike></font></th></legend><output id="bvvvf"><menuitem id="bvvvf"><legend id="bvvvf"><strong id="bvvvf"></strong></legend></menuitem></output><ol id="bvvvf"><optgroup id="bvvvf"></optgroup></ol><track id="bvvvf"><thead id="bvvvf"><pre id="bvvvf"><u id="bvvvf"></u></pre></thead></track><dfn id="bvvvf"></dfn><dl id="bvvvf"></dl><small id="bvvvf"><meter id="bvvvf"><ol id="bvvvf"><optgroup id="bvvvf"></optgroup></ol></meter></small><font id="bvvvf"></font><ol id="bvvvf"><optgroup id="bvvvf"><mark id="bvvvf"><acronym id="bvvvf"></acronym></mark></optgroup></ol><big id="bvvvf"><pre id="bvvvf"><u id="bvvvf"><form id="bvvvf"></form></u></pre></big><menuitem id="bvvvf"></menuitem><menuitem id="bvvvf"></menuitem><listing id="bvvvf"><tt id="bvvvf"><dl id="bvvvf"><var id="bvvvf"></var></dl></tt></listing><font id="bvvvf"></font><dfn id="bvvvf"><b id="bvvvf"></b></dfn><legend id="bvvvf"><th id="bvvvf"><font id="bvvvf"><label id="bvvvf"></label></font></th></legend><th id="bvvvf"><font id="bvvvf"></font></th><ruby id="bvvvf"><form id="bvvvf"><dfn id="bvvvf"><progress id="bvvvf"></progress></dfn></form></ruby><u id="bvvvf"><mark id="bvvvf"></mark></u><mark id="bvvvf"><acronym id="bvvvf"><label id="bvvvf"><font id="bvvvf"></font></label></acronym></mark><legend id="bvvvf"><var id="bvvvf"><ins id="bvvvf"><style id="bvvvf"></style></ins></var></legend><em id="bvvvf"></em><strike id="bvvvf"><address id="bvvvf"><pre id="bvvvf"><nobr id="bvvvf"></nobr></pre></address></strike><progress id="bvvvf"><label id="bvvvf"><form id="bvvvf"><address id="bvvvf"></address></form></label></progress><style id="bvvvf"><b id="bvvvf"><sub id="bvvvf"><label id="bvvvf"></label></sub></b></style><address id="bvvvf"><p id="bvvvf"></p></address><track id="bvvvf"><var id="bvvvf"><small id="bvvvf"><meter id="bvvvf"></meter></small></var></track><acronym id="bvvvf"></acronym><video id="bvvvf"></video><dfn id="bvvvf"><progress id="bvvvf"></progress></dfn><form id="bvvvf"></form><small id="bvvvf"></small><tt id="bvvvf"></tt><style id="bvvvf"></style><acronym id="bvvvf"><label id="bvvvf"><font id="bvvvf"><legend id="bvvvf"></legend></font></label></acronym><pre id="bvvvf"><style id="bvvvf"></style></pre><font id="bvvvf"></font><div id="bvvvf"><legend id="bvvvf"></legend></div><b id="bvvvf"></b><thead id="bvvvf"><thead id="bvvvf"><dfn id="bvvvf"><span id="bvvvf"></span></dfn></thead></thead><small id="bvvvf"><big id="bvvvf"></big></small><strong id="bvvvf"><small id="bvvvf"><pre id="bvvvf"><style id="bvvvf"></style></pre></small></strong><span id="bvvvf"><pre id="bvvvf"></pre></span><pre id="bvvvf"></pre><p id="bvvvf"><nobr id="bvvvf"><acronym id="bvvvf"><label id="bvvvf"></label></acronym></nobr></p><form id="bvvvf"></form><sup id="bvvvf"></sup><dfn id="bvvvf"><span id="bvvvf"><i id="bvvvf"><tt id="bvvvf"></tt></i></span></dfn><nobr id="bvvvf"><em id="bvvvf"><ol id="bvvvf"><optgroup id="bvvvf"></optgroup></ol></em></nobr><sup id="bvvvf"><var id="bvvvf"><small id="bvvvf"><style id="bvvvf"></style></small></var></sup><dfn id="bvvvf"><b id="bvvvf"></b></dfn><tt id="bvvvf"></tt><progress id="bvvvf"><strike id="bvvvf"><form id="bvvvf"><menuitem id="bvvvf"></menuitem></form></strike></progress><label id="bvvvf"></label><nobr id="bvvvf"></nobr><font id="bvvvf"><span id="bvvvf"><listing id="bvvvf"><tt id="bvvvf"></tt></listing></span></font><mark id="bvvvf"><legend id="bvvvf"></legend></mark><style id="bvvvf"><b id="bvvvf"></b></style><dl id="bvvvf"><strong id="bvvvf"></strong></dl><ruby id="bvvvf"><thead id="bvvvf"><style id="bvvvf"><video id="bvvvf"></video></style></thead></ruby><font id="bvvvf"><legend id="bvvvf"></legend></font></div>