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class="jyms"><p><span>簡(jiǎn)要描述：</span>用亞硫酸氫鹽處理基因組DNA，所有未發(fā)生甲基化的胞嘧啶被轉化為尿嘧啶，而甲基化的胞嘧啶不變；隨后設計針對甲基化和非甲基化序列的引物進(jìn)行PCR。通過(guò)電泳檢測MSP擴增產(chǎn)物，如果用針對處理后甲基化DNA鏈的引物能得到擴增片段，則說(shuō)明該位點(diǎn)存在甲基化；反之，說(shuō)明被檢測的位點(diǎn)不存在甲基化。</p></div> <ul> <li><img src="/Skins/270236/images/dot.png" /><span>產(chǎn)品型號：</span></li> <li><img src="/Skins/270236/images/dot.png" /><span>廠(chǎng)商性質(zhì)：</span>生產(chǎn)廠(chǎng)家</li> <li><img src="/Skins/270236/images/dot.png" /><span>更新時(shí)間：</span>2024-05-13</li> <li><img src="/Skins/270236/images/dot.png" /><span>訪(fǎng) 問(wèn) 量：</span>5460</li> </ul> <div id="im8tiuhso" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2">聯(lián)系我們</a> </div> </div> <div id="im8tiuhso" class="clear"></div> </div>  <div class="im8tiuhso" id="ny_pro_box02"> <div class="im8tiuhso" id="con"> <ul id="tags"> <li id="im8tiuhso" class=selectTag><A onmouseover="selectTag('tagContent0',this)" href="javascript:void(0)"onFocus="this.blur()">產(chǎn)品介紹：</A> </li> </ul> <div class="im8tiuhso" id=tagContent> <div id="im8tiuhso" class="tagContent selectTag" id=tagContent0><p><strong>DNA甲基化修飾檢測服務(wù)</strong></p><p><strong>甲基化特異性的PCR（Methylation-specific PCR，MSP）</strong></p><p>用亞硫酸氫鹽處理基因組DNA，所有未發(fā)生甲基化的胞嘧啶被轉化為尿嘧啶，而甲基化的胞嘧啶不變；隨后設計針對甲基化和非甲基化序列的引物進(jìn)行PCR。通過(guò)電泳檢測MSP擴增產(chǎn)物，如果用針對處理后甲基化DNA鏈的引物能得到擴增片段，則說(shuō)明該位點(diǎn)存在甲基化；反之，說(shuō)明被檢測的位點(diǎn)不存在甲基化。</p><p><strong>亞硫酸氫鹽測序法（Bisulfite sequencing PCR，BSP）</strong></p><p>用亞硫酸氫鹽處理基因組DNA，則未發(fā)生甲基化的胞嘧啶被轉化為尿嘧啶，而甲基化的胞嘧啶不變。隨后設計BSP引物進(jìn)行PCR，在擴增過(guò)程中尿嘧啶全部轉化為胸腺嘧啶，zui后對PCR產(chǎn)物進(jìn)行測序就可以判斷CpG位點(diǎn)是否發(fā)生甲基化稱(chēng)為BSP-直接測序方法。將PCR產(chǎn)物克隆至載體后進(jìn)行測序，可以提高測序成功率，這種方法稱(chēng)為BSP-克隆測序法。</p><p><strong>高分辨率熔解曲線(xiàn)法（High Resolution Melting，HRM）</strong></p><p>在非CpG島位置設計一對針對亞硫酸氫鹽修飾后的DNA雙鏈的引物，這對引物中間的片段包含感興趣的CpG島。若這些CpG島發(fā)生了甲基化，用亞硫酸氫鹽處理后，未甲基化的胞嘧啶經(jīng)PCR擴增后轉變成胸腺嘧啶，而甲基化的胞嘧啶不變，樣品中的GC含量發(fā)生改變，從而導致熔解溫度的變化。</p><p><strong>DNA甲基化送樣要求：</strong></p><p>細胞（≥106 個(gè)）、組織（≥300mg）、血液（≥1ml）、血清（≥1.5ml）等樣品材料，基因組DNA（體積≥20μl，濃度≥50 ng/μl）。</p><p><strong>DNA甲基化提供結果：</strong></p><p>引物序列、實(shí)驗報告、包括MSP產(chǎn)物的電泳圖片；引物序列、測序峰圖、陽(yáng)性克隆質(zhì)粒和實(shí)驗報告；擴增曲線(xiàn)、熔解曲線(xiàn)及實(shí)驗報告。</p><p> </p><p> </p></div> <div class="im8tiuhso" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <a name="order" id="order"></a> <div id="im8tiuhso" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="im8tiuhso" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="DNA甲基化檢測服務(wù)" placeholder="請輸入產(chǎn)品名稱(chēng)" /></div> </li> <li> <h4>您的單位：</h4> <div id="im8tiuhso" 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