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培養基提供細胞營(yíng)養和促使細胞增殖的基礎物質(zhì)</span></div> <div id="im8tiuhso" class="about"> <div class="im8tiuhso" id="newscont"> <h1 class="newsname">培養基提供細胞營(yíng)養和促使細胞增殖的基礎物質(zhì)</h1> <li id="im8tiuhso" class="time">發(fā)布日期：2021-06-01 瀏覽次數：1597 </li> <ul class="dcont"> <div>　　培養基是一種常見(jiàn)的器皿，它是指供給微生物、植物或動(dòng)物（或組織）生長(cháng)繁殖的，由不同營(yíng)養物質(zhì)組合配制而成的營(yíng)養基質(zhì)。一般都含有碳水化合物、含氮物質(zhì)、無(wú)機鹽（包括微量元素）、維生素和水等幾大類(lèi)物質(zhì)。培養基既是提供細胞營(yíng)養和促使細胞增殖的基礎物質(zhì)，也是細胞生長(cháng)和繁殖的生存環(huán)境。培養基種類(lèi)很多，根據配制原料的來(lái)源可分為自然培養基、合成培養基、半合成培養基；根據物理狀態(tài)可分為固體培養基、液體培養基、半固體培養基；根據培養功能可分為基礎培養基、選擇培養基、加富培養基、鑒別培養基等；根據使用范圍可分為細菌培養基、放線(xiàn)菌培養基、酵母菌培養基、真菌培養基等。培養基配成后一般需測試并調節pH，還須進(jìn)行滅菌，通常有高溫滅菌和過(guò)濾滅菌。培養基由于富含營(yíng)養物質(zhì)，易被污染或變質(zhì)。配好后不宜久置。</div><div>　　在制備培養基的過(guò)程中，首先要使用一些玻璃器皿，如試管、三角瓶、培養皿、燒杯和吸管等。這些器皿在使用前都要根據不同的情況，經(jīng)過(guò)一定的處理，洗刷干凈。有的還要進(jìn)行包裝，經(jīng)過(guò)滅菌等準備就緒后，才能使用。</div><div>　　1、新購的玻璃器皿</div><div>　　除去包裝沾染的污垢后，先用熱肥皂水刷洗，流水沖凈，再浸泡于1-2％的工業(yè)鹽酸中數小時(shí)，使游離的堿性物質(zhì)除去，再以流水沖凈。對容量較大的器皿，如大燒瓶、量筒等，洗凈后注入濃鹽酸少許，轉動(dòng)容器使其內部表面均沾有鹽酸，數分鐘后傾去鹽酸，再以流水沖凈，倒置于洗滌架上將水空干，即可使用。</div><div>　　2、用過(guò)的玻璃器皿</div><div>　　凡確無(wú)病原菌或未被帶菌物污染的器皿，使用后可隨時(shí)沖洗，吸取過(guò)化學(xué)試劑的吸管，可先浸泡于清水中，待到一定數量后再集中進(jìn)行清洗。有可能被病原菌污染的器皿，必須經(jīng)過(guò)適當消毒后，將污垢除去，用皂液洗刷，再用流水沖洗干凈。若用皂液未能洗凈的器皿，可用洗液浸泡適當時(shí)間后再用清水洗凈。洗液的主要成份是重鉻酸鉀和濃流酸，其作用是將有機物氧化成可溶性物質(zhì)，以便沖洗。洗液有很強的腐蝕作用，使用時(shí)應特別小心，避免濺到衣服、身體和其他物品上。</div> 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